Recombinant Human Epiregulin

名称：Recombinant Human Epiregulin

货号：UN526

规格：5μg/25μg/50μg/100μg/250μg/500μg/1mg

纯度：>97%

系统：E.coli

产品详情

 Recombinant Human Epiregulin

Epiregulin encoded by the EREG gene in humans, is a member of the EGF family of growth factors. This family also includes epidermal growth factor (EGF) , transforming growth factor (TGF) -alpha, amphiregulin (ARG) , HB (heparin-binding) -EGF, betacellulin, and the various Heregulin. Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0kDa mature secreted sequence. Recombinant Human Epiregulin is a 5.6kDa monomeric protein, containing 49 amino residues, which corresponds to the mature secreted Epiregulin sequence.

 名称缩写/Abbreviation：

rHuEpiregulin

货号/Item No.：

UN526

别名/Synonyms：

EREG

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 49 amino acids.

SCI的检索号/Accession #：

O14944 Val60-Leu108

蛋白序列/A.A. Sequence：

VAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuEpiregulin protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.