Recombinant Human Epithelial Neutrophil Activating Peptide-78/CXCL5

名称：Recombinant Human Epithelial Neutrophil Activating Peptide-78/CXCL5

货号：UN200

规格：5μg/20μg/50μg/100μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Epithelial Neutrophil Activating Peptide-78/CXCL5

CXCL5 is a small cytokine also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) . The protein is produced following stimulation of cells with the inflammatory cytokine interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (5-78 a.a.) contains 74 amino acids and it is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57% amino acid sequence identity with mouse and rat CXCL5. Recombinant Human ENA-78/CXCL5 is an 8.1kDa protein containing 74 amino acid residues.

 名称缩写/Abbreviation：

rHuENA-78/CXCL5

货号/Item No.：

UN200

别名/Synonyms：

ENA-78 (5-78), CXCL5 (5-78), Neutrophil Activating Peptide ENA-78, Small-inducible cytokine B5

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

SCI的检索号/Accession #：

XP_004038864 Ala41-Asn114

蛋白序列/A.A. Sequence：

AAVLRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 100 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of r HuENA-78/CXCL5 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY