Recombinant Human Epidermal Growth Factor,1-51a.a

名称：Recombinant Human Epidermal Growth Factor,1-51a.a

货号：UN036B

规格：100μg/500μg/1mg

纯度：>98%

系统：Yeast

产品详情

Recombinant Human Epidermal Growth Factor, 1-51a.a

Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. It was originally discovered in the submaxillary glands of mice and in human urine, and then has been found in many human tissues. EGF stimulates the proliferation of various epidermal and epithelial cells, inhibits gastric secretion, and involves in wound healing. Mature human EGF shares 70% a.a.. sequence identity with mature mouse and rat EGF. Recombinant human EGF,1-51a.a. is a single non-glycosylated polypeptide chain containing   51 amino acids.，there are 3 intramolecular disulfide bonds in the EGF polypeptide chain and the molecular weight of EGF is 6.0kDa.

名称缩写/Abbreviation：  

rHuEGF,1-51a.a.

货号/Item No.：  

UN036B

别名/Synonyms：  

Epidermal Growth Factor, Urogastrone, URG

表达系统/Source：  

Yeast-derived.

分子量/Molecular Weight：  

Approximately 6.0 kDa, a single glycosylated polypeptide chain containing 51 amino acids.

SCI的检索号/Accession #：  

P01133 Asn971-Arg1023

蛋白序列/A.A. Sequence：  

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE

纯度/Purity：  

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁶ IU/mg

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuEGF,1-51a.a. protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.