Recombinant Human Epidermal Growth Facto

名称：Recombinant Human Epidermal Growth Facto

r货号：UN036

规格：100μg/500μg/1mg/50mg

纯度：>95%

系统：E.coli.

产品详情

Recombinant Human Epidermal Growth Factor

Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. It was originally discovered in the submaxillary glands of mice and in human urine, and then has been found in many human tissues. EGF stimulates the proliferation of various epidermal and epithelial cells, inhibits gastric secretion, and involves in wound healing. Recombinant human EGF is a single non-glycosylated polypeptide chain containing   53 amino acids.，there are 3 intramolecular disulfide bonds in the EGF polypeptide chain and the molecular weight of EGF is 6.2kDa.

名称缩写/Abbreviation：  

rHuEGF

货号/Item No.：  

UN036

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 6.2 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.

SCI的检索号/Accession #：  

P01133 Asn971-Arg1023

蛋白序列/A.A. Sequence：  

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

纯度/Purity：  

＞95% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁶ IU/mg

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuEGF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.