Recombinant Human Ciliary Neurotrophic Factor

名称：Recombinant Human Ciliary Neurotrophic Factor

货号：UN219

规格：5μg/20μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Ciliary Neurotrophic Factor

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. The protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant Human CNTF is a 22.9kDa globular protein containing 199 amino acid residues.

名称缩写/Abbreviation：

rHuCNTF

货号/Item No.：

UN219

别名/Synonyms：

Ciliary neurotrophic factor; CNTF; HCNTF

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.

SCI的检索号/Accession #：

P26441 Ala2-Met200

蛋白序列/A.A. Sequence：

AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTQSYVKHQGLNKNINLDSADGMPVASTDQWSQLTQAQRLQQNLQAYRTFHVLLARLLQDQQVHFTPTQGDFHQAIHTLLLQVAAFAYQIQQLMILLQYKIPRNQADGMPINVGDGGLFQKKLWGLKVLQQLSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of ≥ 6.7×10³ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 3% (v/v) Trehalose, 0.02% (v/v) Tween-20, pH 7.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuCNTF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.