Recombinant Human Cerebral Dopamine Neurotrophic Factor

名称：Recombinant Human Cerebral Dopamine Neurotrophic Factor

货号：UN304

规格：5μg/20μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Cerebral Dopamine Neurotrophic Factor

Cerebral dopamine neurotrophic factor (CDNF) , also known as ARMET-like protein 1, is a protein encoded by the CDNF gene and it is widely expressed in neuronal and non-neuronal tissues. The cerebral dopamine neurotrophic factor (CDNF) also is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF) . By research, CDNF prevents the 6-hydroxydopamine (6-OHDA) -induced degeneration of dopaminergic neurons and it might be beneficial for the treatment of Parkinson’s disease. Mature human CDNF shares 80%, 84% a.a. identity with mouse, rat CDNF, respectively. Recombinant Human CDNF is an 18.5kDa protein containing 163 amino-acid residues.

名称缩写/Abbreviation：

rHuCDNF

货号/Item No.：

UN304

别名/Synonyms：

Cerebral Dopamine Neurotrophic Factor, ARMETL1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

SCI的检索号/Accession #：

Q49AH0 Gln25-Leu187

蛋白序列/A.A. Sequence：

QGQEAGGRPGADCEVCKEFLNRFYKSLIDRGVNFSLDTIEKELISFCLDTKGKENRLCYYLGATKDAATKILSEVTRPMSVHMPAMKICEKLKKLDSQICELKYEKTLDLASVDLRKMRVAELKQILHSWGEECRACAEKTDYVNLIQELAPKYAATHPKTEL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5-30 μg/mL on a nitrocellulose-coated microplate.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuCDNF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.