Recombinant Diphtheria Toxin Mutant CRM197

名称：Recombinant Diphtheria Toxin Mutant CRM197

货号：UN121

规格：1mg/10mg/100mg/1g

纯度：>96%

系统：E.coli.

产品详情

Recombinant Diphtheria Toxin Mutant CRM197

CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glycine with glutamic acid. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them more immunogenic. CRM197 is utilized as a carrier protein in a number of approved conjugate vaccines for diseases caused by Streptococcus pneumoniae, Haemophilus influenzae b and Neisseria meningitidis. Recombinant CRM197 is a 58.4kDa globular protein containing 535 amino acids.

名称缩写/Abbreviation：

rCRM197

货号/Item No.：

UN121

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 58.4 kDa, a single non-glycosylated polypeptide chain containing 535 amino acids

SCI的检索号/Accession #：

AMV91693 Gly2-Ser536

蛋白序列/A.A. Sequence：

GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKEFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKTQPFLHDGYAVSWNTVEDSIIRTGFQGESGHDIKITAENTPLPIAGVLLPTIPGKLDVNKSKTHISVNGRKIRMRCRAIDGDVTFCRPKSPVYVGNGVHANLHVAFHRSSSEKIHSNEISSDSIGVLGYQKTVDHTKVNSKLSLFFEIKS

纯度/Purity：

＞96% by SDS-PAGE or HPLC, dimers and high molecular weight impurities: <4% by SEC-HPLC.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 0.025% (v/v) Tween-80 pH7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rCRM197 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.