Recombinant Enhanced Green Fluorescent Protein

名称：Recombinant Enhanced Green Fluorescent Protein

货号：UN053

规格：10μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Enhanced Green Fluorescent Protein

The recombinant eGFP was expressed and purified from transformed E. coli using a method that ensures high purity and maximal eGFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea Victoria, though many other organisms have similar proteins. It is a 26.9kDa protein (composed of 238 AA residues) that shows green fluorescence in short-wave light (blue to ultraviolet) . Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

.Product Information

名称缩写/Abbreviation：

reGFP

货号/Item No.：

UN053

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 239 amino acids.

SCI的检索号/Accession #：

ANC98519.1 Met167-Lys405

蛋白序列/A.A. Sequence：

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Green Fluorescent under UV radiation

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of reGFP protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.