Recombinant Cys-Protein G

名称：Recombinant Cys-Protein G

货号：UN156

规格：1mg/5mg/50mg/1g

纯度：>95%

系统：E.coli.

产品详情

Recombinant Cys-Protein G

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcic. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F (ab') fragments of IgG. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology Recombinant Cys-Protein G is a single non-glycosylated polypeptide chain containing   201 amino acids. and there is an N-terminal cysteine for Site-Specific Conjugation.

名称缩写/Abbreviation：  

Cys-Protein G

货号/Item No.：  

UN156

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 21.9 kDa, and it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE.

SCI的检索号/Accession #：  

P19909 Leu298-Glu495 with an N-terminal Cys

蛋白序列/A.A. Sequence：  

CLPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE

Concentration for 1 unity DO at 280 nm：  

0.729mg/ml

纯度/Purity：  

＞95% by SDS-PAGE or HPLC.

活性/Biological Activity：  

IgG binding activity.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PB, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

浓度/Concentration：  

Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

内毒素/Endotoxin：  

< 0.1 EU/μg of Cys-Protein G protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.