Recombinant Bovine Enterokinase Light Chain,His-Tagged

名称；Recombinant Bovine Enterokinase Light Chain,His-Tagged

货号：UNO21

规格：100U/250U/1KU

纯度：>98%

系统：E.coli

产品详情

Recombinant Bovine Enterokinase Light Chain, His-Tagged

Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. Recombinant EK was reported to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. Recombinant bovine EK has higher activity than EK from other species and is used wildly in biochemical applications. rBoEK with a C-terminal 6×His tag is able to bind to Ni2+ affinity resin and then was removed from the digestion reaction system.

Advantage:

1)produced under GMP-like condition

2)good consistency between lots

3)high purity

4)safe and stable supply

名称缩写/Abbreviation：

rBoEK,His

货号/Item No.：

UN021

别名/Synonyms：

Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 28.0 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids, with a 6×His tag at C-terminus.

蛋白序列/A.A. Sequence：

IVGGSDSREGAWPWVVALYFDDQQVCGASLVSRDWLVSAAHCVYGRNMEPSKWKAVLGLHMASNLTSPQIETRLIDQIVINPHYNKRRKNNDIAMMHLEMKVNYTDYIQPICLPEENQVFPPGRICSIAGWGALIYQGSTADVLQEADVPLLSNEKCQQQMPEYNITENMVCAGYEAGGVDSCQGDSGGPLMCQENNRWLLAGVTSFGYQCALPNRPGVYARVPRFTEWIQSFLHLEHHHHHH

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

≥ 40 KIU/mg.

活性单位定义/Unit Definition：

One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2

配方/Formulation：

A 0.2 μm filtered concentrated solution in 50 mM Tris-HCl, with 500 mM NaCl and 50% (v/v) Glycerol,pH 8.0.

外观/Appearance：

Sterile colorless liquid.

浓度/Concentration：

No less than 10mg/ml, The concentration for a specific batch as shown in the COA

内毒素/Endotoxin：

< 0.1 EU/μg of rBoEK protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.