Recombinant Rat Ciliary Neurotrophic Factor

名称：Recombinant Rat Ciliary Neurotrophic Factor

货号：UN221

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Rat Ciliary Neurotrophic Factor

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Rat CNTF shares 83% and 95% a.a. sequence identity with human and murine CNTF. Recombinant rat CNTF is a 22.7kDa globular protein containing 199 amino acid.

 名称缩写/Abbreviation：

rRtCNTF

货号/Item No.：

UN221

别名/Synonyms：

Ciliary neurotrophic factor;

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.

SCI的检索号/Accession #：

P20294 Ala2-Met200

蛋白序列/A.A. Sequence：

AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of ≥ 3.3×10⁴ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 300 mM NaCl, 3% (v/v) Trehalose, pH 7.2.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rRtCNTF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.