Recomb inant Rat Glial Cell Line-derived Neurotrophic Factor

名称：Recomb inant Rat Glial Cell Line-derived Neurotrophic Factor

货号：UN320

规格：2μg/10μg/50μg/100μg/250μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Rat Glial Cell Line-derived Neurotrophic Factor

Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. Recombinant rat GDNF is a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains and it shares 99% and 93% a.a. sequence identity with murine and human GDNF.

 名称缩写/Abbreviation：

rRtGDNF

货号/Item No.：

UN320

别名/Synonyms：

Glial-Derived Neurotrophic Factor, ATF-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

SCI的检索号/Accession #：

Q07731 Ser78-Ile211

蛋白序列/A.A. Sequence：

SPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁶ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 0.05% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rRtGDNF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.