Recomb inant Streptolysin 0

名称：Recomb inant Streptolysin 0

货号：UN451

规格：20μ/100μg/250μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Streptolysin O

The sulfhydryl-activated toxin acts as a precision "molecular scythe," specifically designed for efficient and targeted cell lysis. It swiftly locates itself on cholesterol-containing host membranes, and upon activation through its unique sulfhydryl mechanism, it undergoes a dramatic conformational shift. This shift enables the toxin to embed itself into the membrane and assemble into a powerful pore complex, leading to the immediate rupture of the target cell. The toxin's specificity is further refined by cholesterol, which not only serves as its entry "passport" but also acts as the critical fuel driving its destructive power. Remarkably, its activity can be reversibly "sealed" through oxidation, allowing for an on-demand activation switch that provides unparalleled convenience and safety in experimental settings.

名称缩写/Abbreviation：  

rStreptolysin O

货号/Item No.：  

UN451

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 60.1 kDa, a single non-glycosylated polypeptide chain containing 538 amino acids.

SCI的检索号/Accession #：  

P0DF97 Asn34-Lys571

蛋白序列/A.A. Sequence：  

NKQNTASTET TTTNEQPKPE SSELTTEKAG QKTDDMLNSN DMIKLAPKEM PLESAEKEEK KSEDKKKSEE DHTEEINDKI YSLNYNELEV LAKNGETIEN FVPKEGVKKA DKFIVIERKK KNINTTPVDI SIIDSVTDRT YPAALQLANK GFTENKPDAV VTKRNPQKIH IDLPGMGDKA TVEVNDPTYA NVSTAIDNLV NQWHDNYSGG NTLPARTQYT ESMVYSKSQI EAALNVNSKI LDGTLGIDFK SISKGEKKVM IAAYKQIFYT VSANLPNNPA DVFDKSVTFK ELQRKGVSNE APPLFVSNVA YGRTVFVKLE TSSKSNDVEA AFSAALKGTD VKTNGKYSDI LENSSFTAVV LGGDAAEHNK VVTKDFDVIR NVIKDNATFS RKNPAYPISY TSVFLKNNKI AGVNNRTEYV ETTSTEYTSG KINLSHQGAY VAQYEILWDE INYDDKGKEV ITKRRWDNNW YSKTSPFSTV IPLGANSRNI RIMARECTGL AWEWWRKVID ERDVKLSKEI NVNISGSTLS PYGSITYK

纯度/Purity：  

＞97% by SDS-PAGE or HPLC.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rStreptolysin O protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.