Recombinant Tetanus Toxin C Fragment

名称：Recombinant Tetanus Toxin C Fragment

货号：UN120

规格：1mg/10mg/100mg/1g

纯度：>96%

系统：E.coli.

产品详情

Recombinant Tetanus Toxin C Fragment

Tetanus toxin is a potent neurotoxin secreted by the anaerobic bacterium Clostridium tetani and is the compound responsible for the symptoms of tetanus. The toxin consists of two polypeptide chains connected by a disulfide linkage. The heavy chain contains two regions of interest: a binding domain and a translocation domain. The binding domain allows the toxin to be taken up by neurons upon binding to gangliosides on nerve endings. Upon reaching inhibitory neurons via axonal and transsynaptic transport, the translocation domain allows the toxin to enter the cytosol. The light chain is a zinc endopeptidase, activated by enzymes in the cytosol that cleaves the disulfide bond between the heavy and light chain. Once activated, the light chain cleaves synaptobrevin 2, blocking release of inhibitory transmitters GABA and glycine to motor neurons, causing the prolonged muscle transactions symptomatic of tetanus. Enzymatic cleavage of tetanus toxin by papain generates two main molecules: 1) the light chain linked to the N-terminal portion of the heavy chain containing the translocation domain, and 2) the C-fragment, or the C-terminal portion of the heavy chain, which contains the binding domain. C-fragment is considered nontoxic and, when recombinantly produced, contains no trace of intact toxin.

In vitro, tetanus toxin C-fragment (TTC) is an efficient mucosal adjuvant and carrier molecule for the generation of mucosal antibody responses and/or induction of systemic T-cell tolerance to linked antigens. TTC is also used for tract tracing in neurological research, taking advantage of its high affinity to GT1B gangliosides and retrograde transport. Recombinant tetanus toxin C-fragment is a 51.6kDa globular protein containing 451 amino acids.

 名称缩写/Abbreviation：

rTTc

货号/Item No.：

UN120

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 51.6 kDa, a single non-glycosylated polypeptide chain containing 451 amino acids.

SCI的检索号/Accession #：

7BY5_A Lys872-Asp1322

蛋白序列/A.A. Sequence：

KNLDCWVDNEEDIDVILKKSTILNLDINNDIISDISGFNSSVITYPDAQLVPGINGKAIHLVNNESSEVIVHKAMDIEYNDMFNNFTVSFWLRVPKVSASHLEQYGTNEYSIISSMKKHSLSIGSGWSVSLKGNNLIWTLKDSAGEVRQITFRDLPDKFNAYLANKWVFITITNDRLSSANLYINGVLMGSAEITGLGAIREDNNITLKLDRCNNNNQYVSIDKFRIFCKALNPKEIEKLYTSYLSITFLRDFWGNPLRYDTEYYLIPVASSSKDVQLKNITDYMYLTNAPSYTNGKLNIYYRRLYNGLKFIIKRYTPNNEIDSFVKSGDFIKLYVSYNNNEHIVGYPKDGNAFNNLDRILRVGYNAPGIPLYKKMEAVKLRDLKTYSVQLKLYDDKNASLGLVGTHNGQIGNDPNRDILIASNWYFNHLKDKILGCDWYFVPTDEGWTND

纯度/Purity：

＞96% by SDS-PAGE or HPLC, dimers and high molecular weight impurities: <5% by SEC-HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured in a hemagglutination assay using sheep red blood cells coated with GT1B ganglioside, and exhibits comparable binding activity as standard.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rTTc protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.