Recombinant Streptavidin

名称：Recombinant Streptavidin

货号：UN001

规格：1mg/10mg/100mg/1g

纯度：>98%

系统：E.coli.

产品详情

Recombinant Streptavidin

Streptavidin is a biotin-binding protein that was originally isolated from Streptomyces avidinii. Streptavidin is superior to avidin because it has almost no net charge at neutral pH and does not contain carbohydrate and exhibits lower level of non-specific binding. Recombinant streptavidin is a tetrameric protein with a molecular weight of approximately 53kDa, consisting of four subunits of approximately 13kDa. Each subunit contains a single biotin-binding site and has six tyrosine. The purified recombinant streptavidin can be applied in detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western Blotting, ELISA, immunohistochemistry and fluorescence imaging.

名称缩写/Abbreviation：  

rSA

货号/Item No.：  

UN001

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

13.3 kDa for monomer, 53.0 kDa for tetramer.

SCI的检索号/Accession #：  

P22629 Ala37-Ser163 with an N-terminal Met

蛋白序列/A.A. Sequence：  

AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGT ALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS

纯度/Purity：  

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Binding capacity: ≥14 μg biotinylated IgG/mg.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM potassium phosphate buffer pH 6.5.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg rSA protein as determined by LAL method.

稳定性和存储/Stability & Storage：  

No observed loss of activity after 10 times repeated freezing and thawing. For long term storage, the product should be stored ≤ -20℃ or below.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.