Recombinant Sortase A

名称：Recombinant Sortase A

货号：UN103

规格：10μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Sortase A

Sortase A (SrtA) is an enzyme obtained from Staphylococcus aureus that catalyzes site-specific transpeptidation of surface proteins to the bacterial cell membrane. SrtA recognizes an LPXTG amino acid motif and cleaves between threonine and Glycine to form a thioester-linked acyl–enzyme intermediate. The intermediate is resolved in the presence of a nucleophilic N-terminal polyglycine resulting in ligation of the acyl donor to the polyglycine acceptor. The ability of SrtA to form amide bonds has been utilized for a wide range of protein and peptide engineering applications, including N- and C-terminal labeling, peptide PEGylation, and the production of macrocycles, where peptides are strategically designed to contain the polyglycine nucleophile at the N-terminus, and the LPXTG “sortagging” motif at the C-terminus. The recombinant SrtAΔN59 is a single peptide chain containing 148 amino acids..

 名称缩写/Abbreviation：

rSrtAΔN59

货号/Item No.：

UN103

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

SCI的检索号/Accession #：

P20809 Pro22-Leu199 with an N-terminal Met

蛋白序列/A.A. Sequence：

MQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 5%Trehalose, 0.02% (v/v) Tween-20, pH 7.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rSrtAΔN59 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.