Recombinant Red Fluorescent Protein

名称：Recombinant Red Fluorescent Protein

货号：UN054

规格：10μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Red Fluorescent Protein

The recombinant RFP was expressed and purified from transformed E. coli using a method that ensures high purity and maximal RFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a Homotetramer containing four 25.93kDa monomers with Ex./Em. = 558/583 nm, extinction coefficient ( 280 nm = 34380 M -1 Cm-1) . The protein is suitable as control reagent for drFP583 expression studies or as labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis, label other proteins, calibration of fluorometers, flow cytometers, and fluorescence microscope, as well as microinjection of drFP583 into cells and tissues, etc. The recombinant drFP583 is ideal for fusion tag applications and is perfect for dual labeling with EGFP

.Product Information

 名称缩写/Abbreviation：

drFP583

货号/Item No.：

UN054

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 225 amino acids.

SCI的检索号/Accession #：

Q9U6Y8 Met1-Leu225

蛋白序列/A.A. Sequence：

MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Red Fluorescent under UV radiation

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 0.02% (v/v) Tween-20,pH 7.4 .

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of drFP583 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.