Recombinant Murine Interleukin-1 alpha

名称：Recombinant Murine Interleukin-1 alpha

货号：UN223

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-1 alpha

IL-1α is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant murine IL-1α is a 17.9kDa protein containing 156 amino acid.

 名称缩写/Abbreviation：

rMuIL-1α

货号/Item No.：

UN223

别名/Synonyms：

Lymphocyte-activating factor, LAF, Leukocyte Endogenous Mediator, LEM, Endogenous Pyrogen, EP, Mononuclear Cell Factor, MCF, Hematopoietin-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

SCI的检索号/Accession #：

Q3U0Y6 Ser115-Ser270

蛋白序列/A.A. Sequence：

SAPYTYQSDLRYKLMKLVRQKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 2.0 pg/ml, corresponding to a specific activity of ≥ 5.0×10⁸ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuIL-1α protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.