Recombinant Murine Inter leukin-1 Receptor Antagonist Protein

名称：Recombinant Murine Inter leukin-1 Receptor Antagonist Protein

货号：UN225

规格：20μg/100μg/500μg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-1 Receptor Antagonist Protein

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The murine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids. and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α. It also shares 90% a.a. sequence identity with rat IL-1RA. Recombinant murine IL-1RA is a 17.3kDa protein containing 152 amino acid.

 名称缩写/Abbreviation：

rMuIL-1RA

货号/Item No.：

UN225

别名/Synonyms：

IL-1RN, IRAP

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

SCI的检索号/Accession #：

Q542W1 Arg8-Gln159

蛋白序列/A.A. Sequence：

RPSGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNIKLEEKIDMVPIDLHSVFLGIHGGKLCLSCAKSGDDIKLQLEEVNITDLSKNKEEDKRFTFIRSEKGPTTSFESAACPGWFLCTTLEADRPVSLTNTPEEPLIVTKFYFQEDQ

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁴ IU/mg in the presence of 50 pg/ml rHuIL-1α.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rMuIL-1RA protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.