Recombinant Murine Interleukin-1 beta

名称：Recombinant Murine Interleukin-1 beta

货号：UN224

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-1 beta

Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1β shares 90% amino acid sequence identity with cotton rat and rat and 65%-78% identity with canine, human and rhesus IL1β. Recombinant murine IL-1β is a 17.5kDa protein containing 152 amino acid.

 名称缩写/Abbreviation：

rMuIL-1β

货号/Item No.：

UN224

别名/Synonyms：

Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, Endogenous Pyrogen, EP

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

SCI的检索号/Accession #：

P10749 Val118-Ser269

蛋白序列/A.A. Sequence：

VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of ≥ 5.0×10⁸ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rMuIL-1βprotein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.