Recombinant Human Platelet-derived Growth Factor-AA

名称：Recombinant Human Platelet-derived Growth Factor-AA

货号：UN125

规格：2μg/10μg/50μg/100μg/250μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Platelet-derived Growth Factor-AA

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant Human PDGF-AA is a 28.8kDa disulfide-linked homodimer of two A chains (252 total amino acids.) .

 名称缩写/Abbreviation：

rHuPDGF-AA

货号/Item No.：

UN125

别名/Synonyms：

Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 28.8 kDa, a disulfide-linked homodimeric protein containing two 126 amino acid polypeptide chains. It migrates with an apparent molecular mass of 33.6 kDa.

SCI的检索号/Accession #：

P04085 Ser87-Thr211 with an N-terminal Met

蛋白序列/A.A. Sequence：

MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 300 mM NaCl, pH 6.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuPDGF-AA protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.