Recombinant Human Platelet-derived Growth Factor-BB

名称：Recombinant Human Platelet-derived Growth Factor-BB

货号：UN026

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Platelet-derived Growth Factor-BB

Platelet-derived growth factors (PDGFs) are dimeric glycoprotein consisting of two 12.0-13.5 kDa polypeptide chains-chain A and chain B. The three naturally occurring subforms, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. PDGF is involved in various biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGFs signal through two receptors, PDGFR-α and PDGFR-β. Recombinant Human PDGF-BB is a 24.3kDa disulfide-linked homodimer of twoβchains, the βchain is a single non-glycosylated polypeptide chain containing   110 amino acids..

名称缩写/Abbreviation：  

rHuPDGF-BB

货号/Item No.：  

UN026

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 24.8 kDa, a single non-glycosylated polypeptide chain containing 110 amino acids.

SCI的检索号/Accession #：  

P01127 Ser82-Thr190 with an N-terminal Met

蛋白序列/A.A. Sequence：  

MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

纯度/Purity：  

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of ≥ 3.3×10⁵ IU/mg protein

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.1 EU/μg of rHuPDGF-BB protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.