Recombinant Human Oncostatin-M,196a.a.

名称：Recombinant Human Oncostatin-M,196a.a.

货号：UN519

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Oncostatin-M, 196a.a.

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi’s sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells. OSM shares several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. The human OSM gene encodes for a 252 amino acid polypeptide, containing 25 amino acid signal sequence for secretion and a 227-precursor protein. Proteolytic processing of this precursor removes an 18 amino acid C-terminal peptide, and generates the mature OSM form. It has approximately 46% and 45% amino acid sequence identity with murine and rat OSM. Recombinant human OSM contains 196 amino acids and has a molecular mass of 22.2kDa.

名称缩写/Abbreviation：

rHuOSM, 196a.a.

货号/Item No.：

UN519

别名/Synonyms：

Oncostatin M; Oncostatin-M; OSM

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 22.2 kDa, a single non-glycosylated polypeptide chain containing 196 amino acids.

SCI的检索号/Accession #：

P13725 Lys26-Arg221

蛋白序列/A.A. Sequence：

AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRR

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁶ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuOSM,196a.a. protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.