Recombinant Human Osteoprotegerin

名称：Recombinant Human Osteoprotegerin

货号：UN273

规格：10μg/50μg/100μg/250μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Osteoprotegerin

Osteoprotegerin (OPG) , also named osteoclastogenesis inhibitory factor (OCIF) , and tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) , is a TNFRSF11B-encoded protein in humans. OPG is a 401 a.a. basic glycoprotein which comprises 7 structural domains. It is either a 60kDa monomer or a 120kDa dimer linked by disulfide bridges. OPG acts as a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL) and inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro and may also play a role in preventing arterial calcification. OPG has been applied to decrease bone resorption in women with postmenopausal osteoporosis and in patients with lytic bone metastases. Recombinant Human OPG is a 19.7kDa non-glycosylated protein containing 173 amino acid residues.

名称缩写/Abbreviation：  

rHuOPG

货号/Item No.：  

UN273

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

SCI的检索号/Accession #：  

O00300 Glu22-Lys194

蛋白序列/A.A. Sequence：  

ETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQK

纯度/Purity：  

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁵ IU/mg in the presence of 10 ng/mL soluble rHuRANKL (sRANKL).

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 6.0.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuOPG protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.