Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3

名称：Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3

货号：UN299

规格：2μg/10μg/50μg/100μg/250μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3

Novel Neurotrophin-1 (NNT-1) or B-Cell Stimulating Factor-3 (BSF-3) , is a new member of the IL-6 family of structurally related cytokines that includes IL-6, CNTF, LIF, CT-1, IL-11 and OSM. All family members share the receptor subunit gp130 that belong to the type I cytokine receptor superfamily. Ligand binding leads to gp130 homodimerization or heterodimerization (with LIF receptor or OSM receptor beta) , and induces cell signaling and functional activity. For several family members, including CNTF, IL-6, and IL-11, binding of the ligand to a specific receptor alpha subunit (CNTF R alpha, IL-6 R alpha, or IL-11 R alpha) is required prior to gp130 homo- or hetero-dimerization. Recombinant human NNT-1/BCSF-3 a 22.3kDa protein containing 198 amino-acid residues.

名称缩写/Abbreviation：

rHuNNT-1/BCSF-3

货号/Item No.：

UN299

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 22.3 kDa, a single non-glycosylated polypeptide chain containing 198 amino acids.

SCI的检索号/Accession #：

Q9UBD9 Leu28-Phe225

蛋白序列/A.A. Sequence：

LNRTGDPGPGPSIQKTYDLTRYLEHQLRSLAGTYLNYLGPPFNEPDFNPPRLGAETLPRATVDLEVWRSLNDKLRLTQNYEAYSHLLCYLRGLNRQAATAELRRSLAHFCTSLQGLLGSIAGVMAALGYPLPQPLPGTEPTWTPGPAHSDFLQKMDDFWLLKELQTWLWRSAKDFNRLKKKMQPPAAAVTLHLGAHGF

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of ≥ 2.5×10⁵ IU/mg in the presence of human CNTF R alpha

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuNNT-1/BCSF-3 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.