Recombinant Human Interleukin-19

名称：Recombinant Human Interleukin-19

货号：UN087

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>95%

系统：E.coli.

产品详情

 Recombinant Human Interleukin-19

Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis through TNF-α. Human and murine IL-19 share 71% amino acid sequence identity. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. In solution, IL-19 exists predominantly as a non-disulfide-linked dimer. Recombinant Human IL-19 is a 17.9kDa globular protein containing 153 amino acid.

 名称缩写/Abbreviation：

rHuIL-19

货号/Item No.：

UN087

别名/Synonyms：

Interleukin-19, Melanoma differentiation association like protein, MDA1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

SCI的检索号/Accession #：

Q9UHD0 Leu25-Ala177

蛋白序列/A.A. Sequence：

LRRCLISTDMHHIEESFQEIKRAIQAKDTFPNVTILSTLETLQIIKPLDVCCVTKNLLAFYVDRVFKDHQEPNPKILRKISSIANSFLYMQKTLRQCQEQRQCHCRQEATNATRVIHDNYDQLEVHAAAIKSLGELDVFLAWINKNHEVMSSA

纯度/Purity：

＞95% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of ≥ 6.7×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-19 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.