Recombinant Human Interleukin-17A

名称：Recombinant Human Interleukin-17A

货号：UN067

规格：5μg/25μg/50μg/100μg/500μg/1mg

纯度：>95%

系统：E.coli.

产品详情

Recombinant Human Interleukin-17A

The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 132 amino acid chains that are secreted by activated T-cells, which act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. The six known members of this family are secreted as homodimers. Human IL-17 exhibits 63% amino acid identity with mouse IL-17，IL-17A exhibits cross-species bioactivity between human and murine cells. Recombinant Human IL-17A is a 31.0kDa, disulfide-linked homodimer of two 132 amino acid polypeptide chains.

 名称缩写/Abbreviation：

rHuIL-17A

货号/Item No.：

UN067

别名/Synonyms：

IL-17, CTLA-8

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 31.0 kDa, a homodimer of two 132 amino acid polypeptide chains.

SCI的检索号/Accession #：

Q16552 Gly24-Ala154 with a N-terminal Met

蛋白序列/A.A. Sequence：

MGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA

纯度/Purity：

＞95% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of ≥ 1.3×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-17A protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.