Recombinant Human Interleukin-18

名称：Recombinant Human Interleukin-18

货号：UNO86

规格：5μg/10μ/50μ/100μ/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Interleukin-18

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 Rβ to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst. In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis. Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation. A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18. Recombinant IL-18 is a single non-glycosylated polypeptide chain containing 157 amino acids

名称缩写/Abbreviation：  

rHuIL-18

货号/Item No.：  

GH086

别名/Synonyms：  

Iboctadekin; IFN-gamma-inducing factor; IL-1F4; Interleukin-1 gamma;

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.

SCI的检索号/Accession #：  

Q14116 Tyr37-Asp193

蛋白序列/A.A. Sequence：  

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

纯度/Purity：  

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard substance. Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells. The ED₅₀ for this effect is 1.5-15 ng/ml.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuIL-18 protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.