Recombinant Human Heparin-binding EGF-Iike Growth Factor

名称：Recombinant Human Heparin-binding EGF-Iike Growth Factor

货号：UN423

规格：10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Heparin-binding EGF-like Growth Factor

Heparin-binding epidermal growth factor (HB-EGF) -like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73% and 76% a.a. sequence identity with murine and rat HB-EGF. Recombinant human HB-EGF is a 9.7kDa protein containing 86 amino acid residues.

 名称缩写/Abbreviation：

rHuHB-EGF

货号/Item No.：

UN423

别名/Synonyms：

HBEGF, DT-R

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

SCI的检索号/Accession #：

Q99075 Asp63-Leu148

蛋白序列/A.A. Sequence：

DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁶ U/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, with 500 mM NaCl, 5% (v/v) Trehalose, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuHB-EGF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.