Recombinant Human I-309/CCL1

名称：Recombinant Human I-309/CCL1

货号：UN166

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

 Recombinant Human I-309/CCL1

Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokine (also known as chemokines) . It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5kDa. CCL1 signals through the CCR8 receptor. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Recombinant Human I-309 is an 8.6kDa protein consisting of 74 amino acid residues that include six cysteine residues, of which four are the highly conserved cysteine residues present in CC chemokines. Unlike some CC chemokines, whose biologically active form in solution is a non-disulfide-linked dimer, the biologically active form of I-309 is monomeric.

 名称缩写/Abbreviation：

rHuI-309/CCL1

货号/Item No.：

UN166

别名/Synonyms：

Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

SCI的检索号/Accession #：

P22362 Ser23-Lys96

蛋白序列/A.A. Sequence：

SKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuI-309/CCL1 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.