Recombinant Human Hemofiltrate CC Chemokine-1/CCL14

名称：Recombinant Human Hemofiltrate CC Chemokine-1/CCL14

货号：UN177

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

 Recombinant Human Hemofiltrate CC Chemokine-1/CCL14

Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3) . The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma. Recombinant human HCC-1/CCL14 is an 8.4kDa protein containing 72 amino-acid residues including the four highly conserved cysteine residues present in the CC chemokines.

 名称缩写/Abbreviation：

rHuHCC-1/CCL14

货号/Item No.：

UN177

别名/Synonyms：

HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.

SCI的检索号/Accession #：

Q16627 Thr22-Asn93

蛋白序列/A.A. Sequence：

TESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRGHSVCTNPSDKWVQDYIKDMKEN

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuHCC-1/CCL14 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.