Recombinant Beta-lactamase TEM-1

名称：Recombinant Beta-lactamase TEM-1

货号：UN153D

规格：1mg纯度：>95%

系统：E.coli.

产品详情

Recombinant Beta-lactamase TEM-1

TEM-1 β-lactamase, a plasmid-mediated class A enzyme, is the most prevalent β-lactamase in Gram-negative bacteria. This 29 kDa monomeric protein (264 amino acids) hydrolyzes β-lactam antibiotics via a serine-active mechanism (Ser70, Lys73, Glu166) without requiring cofactors. Its structure comprises two domains: an N-terminal β-sheet/α-helix fold and a C-terminal α-helical bundle, forming a catalytic pocket. TEM-1 efficiently inactivates penicillins and first-gen cephalosporins (e.g., cephalothin, ~1,850 μmol/min/mg activity for penicillin G), driving ampicillin resistance in 90% of E. coli isolates. Nitrocefin, a chromogenic substrate, enables rapid activity detection (yellow→red).

Purified recombinant TEM-1 (≥90% purity) serves as a critical tool for enzyme kinetics, antibody validation, and inhibitor screening (e.g., clavulanic acid). Its mutants (e.g., TEM-10, TEM-52) with expanded substrate spectra model resistance evolution. Over 174 TEM variants (as of 2022) underpin research on antibiotic resistance and targeted therapies. Ideal for molecular dynamics studies and β-lactamase inhibitor development.

 名称缩写/Abbreviation：

rβLactamase

货号/Item No.：

UN153D

别名/Synonyms：

TEM-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.4 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids.

蛋白序列/A.A. Sequence：

MHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWNMEADKVAGPLLRSALPAGWFIADKSGAGERGSSRGIIAALGPDGKPSSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW

纯度/Purity：

＞95% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. One unit of enzyme activity is defined as th

e amount of enzyme which will hydrolyze 1.0 umol of benzyl peniicillin in presence of EDTA at pH

7.0 and at 25 °C.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 100 mM Tirs, pH 7.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.