Recombinant Human Stem Cell Factor

名称：Recombinant Human Stem Cell Factor

货号:   UN047

规格：2μg/10μg/50μg/100μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Stem Cell Factor

Stem cell factor (SCF) , also known as c-kit ligand (KL) , is a hematopoietic growth factor that binds to the c-KIT receptor. SCF is a primary growth and activation factor for mast cells and eosinophils, and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It exists in both soluble and transmembrane forms, and both are functionally active. Recombinant human SCF is an 18.5 kDa polypeptide containing 164 amino acid residues.

 名称缩写/Abbreviation：

SCF, Hematopoietic growth factor KL, MGF

货号/Item No.：

UN047

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 164 amino acids.

SCI的检索号/Accession #：

P21583 Glu26-Ala189

蛋白序列/A.A. Sequence：

EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5×10⁵ units/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of recombinant SCF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.