Recombinant Human Basic Fibroblast Growth Factor

名称：Recombinant Human Basic Fibroblast Growth Factor

货号：UN003

规格：10μg/50μg/100μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Basic Fibroblast Growth Factor

BFGF, a member of the fibroblast growth factor (FGF) family, is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. bFGF is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. bFGF signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic is a 16.5kDa protein consisting of 146 amino acid.

名称缩写/Abbreviation：  

bFGF

货号/Item No.：  

UN003

别名/Synonyms：  

Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

SCI的检索号/Accession #：  

P09038 Pro143-Ser288 with an N-terminal Met

蛋白序列/A.A. Sequence：  

MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

纯度/Purity：  

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁷ IU/mg.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, with 150 mM NaCl, 5% (v/v) Trehalose, 0.02% (v/v) Tween-20, pH 7.6.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHubFGF protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.