Recombinant Human Fibroblast Growth Factor 21

名称：Recombinant Human Fibroblast Growth Factor 21

货号：UN033

规格：5μg/25μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

 Recombinant Human Fibroblast Growth Factor 21

FGF-21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-21, in the presence of β-Klotho as a protein cofactor, signals through the FGFR 1c and 4 receptors, and stimulates insulin-independent glucose uptake by adipocytes. Mature human FGF-21 respectively shows 81% a.a. identity to murine and rat FGF-21, and is known to be active on murine cells. Recombinant Human FGF-21 is a 19.4kDa protein containing 181 amino acid.

 名称缩写/Abbreviation：

rHuFGF-21

货号/Item No.：

UN033

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

SCI的检索号/Accession #：

Q9NSA1 His29-Ser209

蛋白序列/A.A. Sequence：

HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of ≥ 2.0×10³ IU/mg in the presence of 5 μg/ml of rMuβ-Klotho and 10 μg/ml of heparin.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuFGF-21 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.