Recombinant Human Des1-3 Insulin-like Growth factor-1

名称：Recombinant Human Des1-3 Insulin-like Growth factor-1

货号：UNO40

规格：20μg/100μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Des1-3 Insulin-like Growth factor-1

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity. Des1-3IGF-1, is a truncated variant of human IGF-1 with the tripeptide Gly-Pro-Glu absent from the N-terminus. The des1-3IGF-1 probably results from post-translational cleavage of IGF-1. It has about 10-fold more potent than IGF-1 at stimulating hypertrophy and proliferation of cultured cells, a consequence of much reduced binding to IGF-binding proteins, in turn caused by the absence of the glutamate at position 3. Recombinant human des1-3IGF-1 is a non-glycosylated polypeptide chain containing   67 amino acids..

名称缩写/Abbreviation：  

rHuDes1-3IGF1

货号/Item No.：  

UN040

别名/Synonyms：  

Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 7.4 kDa, a non-glycosylated polypeptide chain containing 67 amino acids.

SCI的检索号/Accession #：  

P05019 Thr49-Ala118

蛋白序列/A.A. Sequence：  

TLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

纯度/Purity：  

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.0.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuDes1-3IGF1 protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.