Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A,Isoform 1-TAT

名称：Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A,Isoform 1-TAT

货号：UN385

规格：5μg/20μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A, Isoform 1-TAT

Cyclin-dependent kinase inhibitors (CDKIs) are proteins that bind to and inhibit the activity of CDKs. Two major classes of CDK inhibitors have been identified. The p16 family (p15, p16, p18 and p19) binds to and inhibits the activities of CDK4 and CDK6. The p21 family (p21, p27, p28 and p57) can bind to broad range of CDK-cyclin complexes and inhibit their activities. CDKIs are capable of suppressing growth, and several lines of evidence strongly suggest that at least some CDKIs may be tumor suppressor proteins. p16-INK4A is the member of p16 family and is encoded by CDKN2A gene in humans. It has three isoforms, which are wildly expressed but not detected in brain or skeletal muscle, except that isoform 3 is pancreas-specific. Full-length human p16INK4a shares 63% aa identity with mouse p16INK4a. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary, as well as transformed, cells. Recombinant Human p16-INK4a-TAT expressed in E. coli is an 18.0kDa protein containing 167 amino-acid residues, including the 155 residues of full-length p16-INK4a and a 12-residue C-terminal TAT peptide (GYGRKKRRQRRR) .

 名称缩写/Abbreviation：

rHuP16-INK4a-TAT

货号/Item No.：

UN385

别名/Synonyms：

CDK4I, MTS-1, p16INK4A

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids.

SCI的检索号/Accession #：

P42771 Met1-Asp156 with a 12-residue C-terminal TAT peptide

蛋白序列/A.A. Sequence：

MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPDGYGRKKRRQRRR

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

配方/Formulation：

A 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, 50% (v/v) Glycerol, pH 7.4.

外观/Appearance：

Sterile colorless liquid.

浓度/Concentration：

No less than 10mg/ml, The concentration for a specific batch as shown in the COA

内毒素/Endotoxin：

< 0.01 EU/μg of rHuP16-INK4a-TAT protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.