Recombinant Benzonase

名称：Recombinant Benzonase

货号：GH039

规格：10μg/100μg/1mg/10mg

纯度：>98%

系统：E.coli

产品详情

Recombinant Benzonase

Benzonase is a nuclease that Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3' position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA. Recombinant benzonase is a 26.8 kDa nuclease consisting of 246 amino acid.

名称缩写/Abbreviation：rBenzonase

货号/Item No.：UN039

表达系统/Source：Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.8 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids.

SCI的检索号/Accession #：P13717 Ala22-Asn266

蛋白序列/A.A. Sequence：

ADTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN

纯度/Purity：＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. 1mg of recombinant benzonase contains activity equivalent to at least 1.5×10⁶ units.

配方/Formulation：

A 0.2 μm filtered concentrated solution in 50 mM Tris, with 20 mM NaCl, 2 mM MgCl2, 50% (v/v) Glycerol, pH 8.0.

外观/Appearance：Sterile colorless liquid.

内毒素/Endotoxin：< 0.01 EU/μg of protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.