Recombinant Apis Mellifera Phosphol ipase A2/Api m1

名称：Recombinant Apis Mellifera Phosphol ipase A2/Api m1

货号：UN503

规格：10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Apis Mellifera Phospholipase A2/Api m1

Api m1, a glycoprotein of the phospholipase 2 (PLA2) family, is a major allergen of Apis mellifera (honeybee) venom (HBV) and a marker allergen for genuine sensitization to this venom. Api m 1 contributes 12-16% of venom dry weight. Api m 1 is consistently found as a major allergen in HBV-allergic patients. Of the 12 known allergens within HBV, Api m 1 is considered the most prominent allergen, in terms of prevalence of sensitization, levels of specific IgE to Api m 1, and quantitative correlation between Api m 1 and HBV specific IgE. Api m 1 does not cross-react with phospholipases of the PLA1 family found in Vespid venoms, therefore, Api m 1 is a marker allergen allowing to discriminate between HBV and Vespid venom sensitization. Recombinant Api m1 is a 15.2kDa globular protein containing 134 amino acid residues.

名称缩写/Abbreviation：rApi m1

货号/Item No.：UN503

别名/Synonyms：PLA2

表达系统/Source：Expressed in E. coli.

分子量/Molecular Weight：

Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.

SCI的检索号/Accession #：P00630 Ile34-Tyr167

蛋白序列/A.A. Sequence：

IIYPGTLWCGHGNKSSGPNELGRFKHTDACCRTHDMCPDVMSAGESKHGLTNTASHTRLSCDCDDKFYDCLKNSADTISSYFVGKMYFNLIDTKCYKLEHPVTGCGERTEGRCLHYTVDKSKPKVYQWFDLRKY

纯度/Purity：＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：Strong Reactivity to honeybee stung positive serum by IGEs test.

配方/Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：< 0.01 EU/μg of rApi m1 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.