Mouse Rnase Inhibitor

货号/Item No.：  

UN113

规格：2KU/10KU/100KU

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 49.8 kDa, a single non-glycosylated polypeptide chain containing 456 amino acids.

SCI的检索号/Accession #：  Q91VI7 Met1-Ser456

蛋白序列/A.A. Sequence：  

MSLDIQCEQLSDARWTELLPLIQQYEVVRLDDCGLTEVRCKDISSAVQANPALTELSLRTNELGDGGVGLVLQGLQNPTCKIQKLSLQNCGLTEAGCGILPGMLRSLSTLRELHLNDNPMGDAGLKLLCEGLQDPQCRLEKLQLEYCNLTATSCEPLASVLRVKADFKELVLSNNDLHEPGVRILCQGLKDSACQLESLKLENCGITAANCKDLCDVVASKASLQELDLSSNKLGNAGIAALCPGLLLPSCKLRTLWLWECDITAEGCKDLCRVLRAKQSLKELSLASNELKDEGARLLCESLLEPGCQLESLWIKTCSLTAASCPYFCSVLTKSRSLLELQMSSNPLGDEGVQELCKALSQPDTVLRELWLGDCDVTNSGCSSLANVLLANRSLRELDLSNNCMGGPGVLQLLESLKQPSCTLQQLVLYDIYWTNEVEEQLRALEEERPSLRIIS

纯度/Purity：  ＞98% by SDS-PAGE or HPLC.

活性单位定义/Unit Definition：  

One unit is defined as the amount of Mouse RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2’, 3’-cyclic monophosphate by RNase A.

推荐使用条件/Recommended Conditions for Cleavage of a Fusion Protein：  

cDNA Synthesis: 40 units/20 μl of reaction mixture,   RNase Inhibitor protects mRNA and improves total cDNA yields including percent total full length of cDNA.

RT-PCR: 40 units/20 μl of reaction mixture.

In Vitro Transcription: 20-40 units/10 μl of reaction mixture,   RNase Inhibitor has been shown to be useful for the isolation of intact RNA transcripts using T3, T7 and SP6 RNA Polymerases.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 50 mM NaCl, pH 7.5 8 mM DTT, 50% Glycerol.

外观/Appearance：  

Sterile colorless liquid.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.