Recombinant Murine Beta-defensin 3/UN448/rMuBD-3

Recombinant Murine Beta-defensin 3 货号 ：UN448 规格 ：5μg/20μg/50μg/100μg/250μg/500μg/1mg

产品详情：

Recombinant Murine Beta-defensin 3 Defensins are small (2-6 kDa), cationic peptides essential for innate immunity. Mammalian defensins are categorized into alpha, beta, and theta based on size and disulfide patterns. Beta-defensins, expressed by leukocytes and epithelial cells, feature a six-cysteine motif forming three intra-molecular disulfide bonds. Their positive charge enables strong interaction with negatively charged microbial membranes (LPS in Gram-negative and LTA in Gram-positive bacteria), leading to membrane disruption and microbial death. Moreover, beta-defensins act as chemokines, attracting monocytes, T-lymphocytes, dendritic cells, and mast cells, thus bridging innate and adaptive immunity.

名称缩写/Abbreviation：   rMuBD-3

货号/Item No.：   UN448

表达系统/Source：   Expressed in E. coli.

分子量/Molecular Weight：   Approximately 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.

SCI的检索号/Accession #：   Q9WTL0 Lys1-Lys41

蛋白序列/A.A. Sequence：   KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K

纯度/Purity：   ＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：   Fully biologically active when compared to standard. The ED₅₀ as determined by anti-microbial activity against E.coli. is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.

配方/Formulation：   Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：   Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：   < 0.01 EU/μg of rMuBD-3 protein as determined by LAL method.

复溶/Reconstitution：   Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：   For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. * THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.