ecombinant Human Vascular Endothelial Growth Factor 121/UN579/rHuVEGF121, Yeast-derived

ecombinant Human Vascular Endothelial Growth Factor 121 货号 :UN579 规格 :2μg/10μg/50μg/100μg/250μg/500μg/1mg

产品详情：

Recombinant Human Vascular Endothelial Growth Factor 121 Recombinant Human Vascular Endothelial Growth Factor 121 (rHuVEGF121) is a disulfide-linked homodimeric protein produced in yeast, with each subunit consisting of 121 amino acids. As a diffusible isoform lacking heparin-binding domains, it exhibits high mobility in the extracellular matrix and circulates more freely than longer VEGF isoforms (e.g., VEGF165). The protein stimulates angiogenesis and vasculogenesis by activating VEGFR-1 and VEGFR-2 receptors, promoting endothelial cell proliferation, migration, and vascular permeability. This variant shares 88% amino acid identity with mouse and rat VEGF, and 93%–96% with porcine, canine, bovine, feline, and equine homologs, highlighting evolutionary conservation. 名称缩写/Abbreviation：   rHuVEGF121, Yeast-derived

货号/Item No.：   UN579

表达系统/Source：   Yeast-derived.

分子量/Molecular Weight：   Approximately 28.2 kDa, a disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains, with N-terminal Met. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 14.4-20 kDa in SDS-PAGE under reducing conditions.

SCI的检索号/Accession #：   P15692-9 Asp27-Arg147

蛋白序列/A.A. Sequence：   APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENCDKPRR

纯度/Purity：   ＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：   Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is between 0.2-0.4 ng/ml.

配方/Formulation：   Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：   Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：   < 0.01 EU/μg of rHuVEGF121 Yeast-derived protein as determined by LAL method.

复溶/Reconstitution：   Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：   For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. * THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.