Recombinant Human TNF Ligand-related Molecule 1/TNFSF1/UN217/rHuTL-1A/TNFSF15/VEGI; VEGI192A; VEGITNF ligand-related molecule 1

Recombinant Human TNF Ligand-related Molecule 1/TNFSF15 

货号： UN217 

规格： 5μg/20μg/50μg/100μg/1mg

产品详情：

Recombinant Human TNF Ligand-related Molecule 1/TNFSF15 TL-1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15) . TL-1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-α and IL-1α. TL-1A binds with high affinity to death receptor 3 (DR3) , known as TNFRSF25. Depending on the cell context, ligation of DR3 by TL-1A can trigger one of two signaling pathways, activation of the transcription factor NF-kB, or activation of caspases and apoptosis. Recombinant Human TL-1A/TNFSF15 is a 20.5kDa globular protein containing 180 amino acid residues.   名称缩写/Abbreviation： rHuTL-1A/TNFSF15

货号/Item No.： UN217

别名/Synonyms： VEGI; VEGI192A; VEGITNF ligand-related molecule 1

表达系统/Source： Expressed in E. coli.

分子量/Molecular Weight： Approximately 20.5 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

SCI的检索号/Accession #： O95150 Leu72-Leu251

蛋白序列/A.A. Sequence： LKGQEFAPSHQQVYAPLRADGDKPRAHLTVVRQTPTQHFKNQFPALHWEHELGLAFTKNRMNYTNKFLLIPESGDYFIYSQVTFRGMTSECSEIRQAGRPNKPDSITVVITKVTDSYPEPTQLLMGTKSVCEVGSNWFQPIYLGAMFSLQEGDKLMVNVSDISLVDYTKEDKTFFGAFLL

纯度/Purity： ＞96% by SDS-PAGE or HPLC.

活性/Biological Activity： Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce apoptosis using human TF-1 cells is less than 20 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁴ IU/mg

配方/Formulation： Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 0.02% (v/v) Tween-20, pH 7.4 .

外观/Appearance： Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin： < 0.01 EU/μg of rHuTL-1A/TNFSF15 protein as determined by LAL method.

复溶/Reconstitution： Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage： For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. * THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.