Recombinant Rat Interleukin-6

名称：Recombinant Rat Interleukin-6

货号：UN034B

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E coli.

产品详情

Recombinant Rat Interleukin-6

Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. The rat IL-6 shares 40% and 85% aa sequence identity with human and mouse IL-6 and it is equally active on human and mouse cells. Recombinant Rat IL-6 is a 21.7kDa protein containing 187 amino acid.

 名称缩写/Abbreviation：

rRtIL-6

货号/Item No.：

UN034B

别名/Synonyms：

BSF-2, CDF, Hybridoma growth factor, IFN-beta-2.

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 187 amino acids.

SCI的检索号/Accession #：

P20607 Phe25-Thr211

蛋白序列/A.A. Sequence：

FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁸ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 5% (v/v) Trehalose, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rRtIL-6 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.