Recombinant Rat Interleukin-33

名称：Recombinant Rat Interleukin-33

货号：UN311

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Rat Interleukin-33

Interleukin-33 (IL-33) , also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Recombinant rat IL-33 is a single non-glycosylated polypeptide chain containing 156 amino acid residues and it shares 59% and 90% a.a. sequence identity with human and murine IL-33.

名称缩写/Abbreviation：  

rRtIL-33

货号/Item No.：  

UN311

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

SCI的检索号/Accession #：  

Q66H70 Ser109-Met264

蛋白序列/A.A. Sequence：  

SIQGTSLLTESCALSTYNDQSVSFVLENGCYVINVEDCGKNQEKDKVLLRYYESSFPAQSGDGVDGKKLMVNMSPIKDTDIWLNANDKDYSVELQKGDVSPPDQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEENDESCNNIMFKLSKM

纯度/Purity：  

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁶ IU/mg.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rRtIL-33 protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.