Recombinant Murine Tumor Necrosis Factor alpha

名称：Recombinant Murine Tumor Necrosis Factor alpha

货号：UN122

规格：5μg/25μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Murine Tumor Necrosis Factor alpha

Tumor necrosis factor alpha (TNF-α) , also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types. The recombinant Murine TNF-α is a non-glycosylated polypeptide chain containing 157 amino acids.

缩写/Abbreviation：

rMuTNF-α

货号/Item No.：

UN122

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.4 kDa, a polypeptide chain containing 157 amino acids. The native form of murine TNF-α is reportedly a trimer.

SCI的检索号/Accession #：

P06804 Leu80-Leu235 with an N-terminal Met

蛋白序列/A.A. Sequence：

MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D，The ED₅₀ for this effect is 10-50 pg/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuTNF-α protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.