Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

名称：Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

货号:   UN479

规格：2μg/10μg/50μg/100μg/250μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7) , is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2) , shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species cross active, murine KGF-1 shares 96% amino acid sequence identity with human and rat. Recombinant murine KGF-1/FGF-7 is an 18.7kDa globular protein containing 163 amino acid residues.

 名称缩写/Abbreviation：

rMuKGF-1/FGF-7

货号/Item No.：

UN479

别名/Synonyms：

HBGF-7

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

SCI的检索号/Accession #：

P36363 Cys32-Thr194

蛋白序列/A.A. Sequence：

CNDMSPEQTATSVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNSYNIMEIRTVAVGIVAIKGVESEYYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHSGGEMFVALNQKGIPVKGKKTKKEQKTAHFLPMAIT

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 800 mM NaCl, pH 8.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuKGF-1/FGF-7 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled PBS or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.5 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.