Recombinant Murine Interleukin-5

名称：Recombinant Murine Interleukin-5

货号：UN227

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-5

IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) , which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. The cDNA for murine IL-5 encodes a signal peptide and a 113 amino acid mature protein. Mature murine IL-5 shares 70% and 94% a.a. sequence identity with human and rat IL5 respectively. It shows cross-reactivity with human IL5 receptor. Recombinant Murine IL-5 is a 26.2kDa disulfide-linked homodimeric protein containing two 113 amino acid chains.

 名称缩写/Abbreviation：

rMuIL-5

货号/Item No.：

UN227

别名/Synonyms：

B-cell differentiation factor I, Eosinophil differentiation factor, TRF

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.2 kDa, a disulfide-linked homodimeric protein containing two 113 amino acids.

SCI的检索号/Accession #：

P04401 Met21-Gly133

蛋白序列/A.A. Sequence：

MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-Cl, 150 mM NaCl, pH 9.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuIL-5 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.