Recombinant Murine Interleukin-4

名称：Recombinant Murine Interleukin-4

货号：UN193

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-4

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The mouse IL-4 has a compact, globular fold, stabilized by three disulfide bonds. The human IL-4 shares about 40% aa sequence identity with mouse/rat IL-4 and they are species-specific in their activities. Recombinant Murine IL-4 is a 13.5kDa globular protein consisting of 121 amino acid residues.

 名称缩写/Abbreviation：

rMuIL-4

货号/Item No.：

UN193

别名/Synonyms：

B-cell IgG differentiation factor, B-cell growth factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.

SCI的检索号/Accession #：

P07750 His21-Ser140 with an N-terminal Met

蛋白序列/A.A. Sequence：

MHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent proliferation of Murine HT-2 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rMuIL-4 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.