Recombinant Murine Interleukin-13

名称：Recombinant Murine Interleukin-13

货号:   UN232

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Murine Interleukin-13

Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human, mouse and rat IL-13 share low homology, but have cross species activity. Recombinant Murine IL-13 is a 12.3kDa protein consisting of 111 amino acid

 名称缩写/Abbreviation：

rMuIL-13

货号/Item No.：

UN232

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids.

SCI的检索号/Accession #：

P20109 Pro22-Phe131 with a N-terminal Met

蛋白序列/A.A. Sequence：

MPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of ≥ 2.5×10⁵ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rMuIL-13 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.